Clinical Applications of NGS in Infectious Diseases - the State of the Art
A Talk by Prof. Shangxin Yang (UCLA Department of Pathology and Laboratory Medicine, CA, USA)
About this Talk
Next-generation sequencing (NGS) has shown tremendous valuable in the clinical setting including hospital outbreak investigations for infection prevention, anti-microbial resistance profiling for guiding therapeutics, and direct pathogen detection for diagnosis. Although NGS has become increasingly available in clinical microbiology, how to best utilize it is an open and evolving question. In this lecture, essential technical background of NGS will be introduced and clinical applications will be demonstrated. The opportunities and limitations of NGS-based infectious disease testing will also be discussed.
After the talk, the audience will be able to:
1. Learn the basic knowledge and nomenclature of NGS and understand the major differences in various NGS methodologies.
2. Understand the principles and difference of targeted metagenomic sequencing vs shotgun metagenomic sequencing.
3. Learn several established and emerging applications of NGS for infectious disease testing and be able to understand the strengths and limitations of NGS.
